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Cellular Model of p21-Induced Senescence

Cellular Model of p21-Induced Senescence

Michael Shtutman, Bey-Dih Chang, Gary P Schools, Eugenia V Broude

Methods Mol Biol. 2017;1534:31-39.

PMID: 27812865

Abstract:

Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Waf1/Cip1/Sdi1.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP367931-A	IPTG		367-93-1	Price

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