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Change in Membrane Permeability Induced by Amyloid Beta-Protein Fragment 25-35 in Brain Neurons Dissociated From Rats

Change in Membrane Permeability Induced by Amyloid Beta-Protein Fragment 25-35 in Brain Neurons Dissociated From Rats

Y Oyama, L Chikahisa, T Ueha, Y Hatakeyama, T Kokubun

Jpn J Pharmacol. 1995 May;68(1):77-83.

PMID: 7494386

Abstract:

Effects of amyloid beta-protein fragment 25-35, A beta P(25-35), on the membrane permeability of organic molecules were examined in the brain neurons dissociated from rats by using an argon laser (equipped in flow cytometer and laser microscope) and a combination of two fluorescent dyes, fluo-3-AM and ethidium bromide. A beta P(25-35) at concentrations of 1 microM or greater induced both leakage of fluo-3 from the neurons and permeation of ethidium across the membrane in a dose-dependent manner, although both dyes are highly impermeant to the intact plasma membrane. Thus, A beta P(25-35) seems to increase not only membrane permeability of inorganic ions such as Ca2+, Na+ and K+, as previously suggested, but also that of organic molecules. Therefore, the brain neuron membrane is suggested to lose its integrity in the presence of A beta P(25-35) that leads to neuronal death.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP131602534	Amyloid β-Protein Fragment 25-35		131602-53-4	Price

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