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Changes in the Mobile Phase Composition on a Stepwise Counter-Current Chromatography Elution for the Isolation of Flavonoids From Siparuna Glycycarpa

Changes in the Mobile Phase Composition on a Stepwise Counter-Current Chromatography Elution for the Isolation of Flavonoids From Siparuna Glycycarpa

Fernanda das Neves Costa, Ian Garrard, Antonio Jorge Ribeiro da Silva, Gilda Guimarães Leitão

J Sep Sci. 2013 Jul;36(14):2253-9.

PMID: 23686916

Abstract:

This paper describes the isolation of flavonoids and other aromatic compounds from an ethyl acetate extract of leaves of Siparuna glycycarpa using stepwise elution counter-current chromatography (CCC). The elution profile yielded the following compounds: diglycosylated flavonoids, quercetin 3-O-rutinoside and quercetin 7-O-rutinoside, followed by monoglycosylated flavonoids, kaempferol-3-O-β-glucopyranoside, kaempferol-3-O-β-rhamnopiranoside, kaempferol-3-O-β-6''(p-coumaroyl) glucopyranoside, and quercetin-3-O-β-glucopyranoside, and then free phenolics, protocatechuic acid, and 2',6'-dihydroxy-4, 4'-dimethoxydihydrochalcone, which shows that this type of elution covers a broader range of polarity than the traditional isocratic mode. This makes it more suitable to perform separations of mixtures containing large differences in hydrophobicity. A GC analysis of a blank CCC run was performed to determine if changes in the mobile phase composition affect the chromatographic process. Results showed a gradual variation of the composition of the mobile phase emerging after the step gradient, favoring the selectivity of the solvent system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP35241544	2′,6′-Dihydroxy 4′,4-dimethoxydihydrochalcone		35241-54-4	Price

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