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Characteristics of Galactose Transport in Saccharomyces Cerevisiae Cells and Reconstituted Lipid Vesicles

J Ramos, K Szkutnicka, V P Cirillo

J Bacteriol. 1989 Jun;171(6):3539-44.

PMID: 2656659

Abstract:

Growth on galactose induces two transport processes, a high-affinity and a low-affinity process. The most important results of a comparison of the two processes were that (i) both depended on GAL2 expression, (ii) only the high-affinity process required galactokinase, (iii) both were down-regulated by catabolite inactivation, (iv) neither was significantly inhibited by carbonyl cyanide-p-trifluoromethoxy-phenyl-hydrazone, (v) neither was differentially inhibited by silver nitrate or mercuric chloride, and (vi) transport activity with a Km closer to that of the low-affinity process of whole cells was reconstituted in fused phospholipid membrane vesicles.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1196157853 Nitrate Ionophore VI Nitrate Ionophore VI 1196157-85-3 Price
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