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Characterization and Antioxidant Activities of Polysaccharides From the Leaves of Lilium Lancifolium Thunb

Zhou Xu, Handong Wang, Bulei Wang, Liang Fu, Ming Yuan, Jing Liu, Lijun Zhou, Chunbang Ding

Int J Biol Macromol. 2016 Nov;92:148-155.

PMID: 27397720

Abstract:

In this study, LLP-1, LLP-2, and LLP-3 three novel polysaccharide fractions were purified from the leaves of Lilium lancifolium, and their physicochemical characterizations and antioxidant properties were investigated by chemical methods, high performance gel permeation chromatography (HPGPC), high performance liquid chromatography (HPLC), fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM), and DPPH radical assay, hydroxyl radical assay, superoxide radical assay and ferrous ion chelating assay, respectively. Results showed that LLP-1, LLP-2, and LLP-3 had low protein and uronic acid contents, meanwhile, their weight-average molecular weight were estimated to be 2.25×106, 2.02×106, and 2.08×106Da, respectively. Mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose glucose and galactose were the major monosaccaherides components presenting in the polysaccharide fractions. Three polysaccharide fractions were not observed triple-helical conformation, while possessed variant surface structure. In addition, three polysaccharide fractions all exhibited significantly scavenging activities against free radical and chelate Fe2+in vitro.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR4243890 LLP-3 LLP-3 Price
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