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Characterization and Enzymatic Hydrolysis of Hydrothermally Treated β-1,3-1,6-glucan From Aureobasidium Pullulans

Characterization and Enzymatic Hydrolysis of Hydrothermally Treated β-1,3-1,6-glucan From Aureobasidium Pullulans

Katsuki Hirabayashi, Nobuhiro Kondo, Sachio Hayashi

World J Microbiol Biotechnol. 2016 Dec;32(12):206.

PMID: 27804105

Abstract:

The chemical structure of hydrothermally treated β-1,3-1,6-glucan from Aureobasidium pullulans was characterized using techniques such as gas chromatography/mass spectrometry (GC/MS) and nuclear magnetic resonance (NMR). The chemical shifts of anomeric carbons observed in the 13C-NMR spectra suggested the presence of single flexible chains of polysaccharide in the sample. β-1,3-1,6-Glucan from A. pullulans became water-soluble, with an average molecular weight of 128,000 Da after hydrothermal treatment, and the solubility in water was approximately 10% (w/w). Sample (3% w/v) was completely hydrolyzed to glucose by enzymatic reaction with Lysing enzymes from Trichoderma harzianum. Gentiobiose (Glcβ1 → 6Glc) and glucose were released as products during the reaction, and the maximum yield of gentiobiose was approximately 70% (w/w). The molar ratio of gentiobiose to glucose after 1 h reaction suggested that the sample is likely highly branched. Sample (3% w/v) was also hydrolyzed to glucose by Uskizyme from Trichoderma sp., indicating that it is very sensitive to enzymatic hydrolysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424807	Lysing Enzymes from Trichoderma harzianum			Price

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