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Characterization and Immobilization of a Novel SGNH Family Esterase ( La SGNH1) from Lactobacillus acidophilus NCFM

Characterization and Immobilization of a Novel SGNH Family Esterase ( La SGNH1) from Lactobacillus acidophilus NCFM

Ly Thi Huong Luu Le, Wanki Yoo, Sangeun Jeon, Kyeong Kyu Kim, T Doohun Kim

Int J Mol Sci. 2019 Dec 21;21(1):91.

PMID: 31877740

Abstract:

The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many Lactobacillus species, was identified, characterized, and immobilized. LaSGNH1 is highly active towards acetate- or butyrate-containing compounds, such as p-nitrophenyl acetate or 1-naphthyl acetate. Enzymatic properties of LaSGNH1, including thermal stability, optimum pH, chemical stability, and urea stability, were investigated. Interestingly, LaSGNH1 displayed a wide range of substrate specificity that included glyceryl tributyrate, tert-butyl acetate, and glucose pentaacetate. Furthermore, immobilization of LaSGNH1 by crosslinked enzyme aggregates (CLEAs) showed enhanced thermal stability and efficient recycling property. In summary, this work paves the way for molecular understandings and industrial applications of a novel SGNH family esterase (LaSGNH1) from Lactobacillus acidophilus.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP60015	Glyceryl tributyrate		60-01-5	Price

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