Home
Resources
Publications
Characterization of a monoPEG20000-Endostar

Characterization of a monoPEG20000-Endostar

Yue Tong, Kai Zhong, Hong Tian, Xiangdong Gao, Xiangyang Xu, Xiaojin Yin, Wenbing Yao

Int J Biol Macromol. 2010 Apr 1;46(3):331-6.

PMID: 20122957

Abstract:

In this study, we investigated the PEG attachment site of mono-PEGylated Endostar, a modified recombinant human endostatin approved in China for lung cancer. N-terminal site-directed mono-PEGylation of Endostar was accomplished using mPEG-propionaldehyde derivatives (Mw=20 kDa) under slightly acidic pH conditions (pH 5.5). One-step cation exchange chromatography was used to purify the mono-PEGylated Endostar. Following tryptic digestion, the peptide fragment containing PEG was separated by SDS-PAGE. Barium iodide staining and Western blotting were used to detect the PEG moiety and the N-terminus of Endostar, respectively. The peptide fragment stained by barium iodide showed a positive response to anti-(His) 6 mAb, demonstrating that PEG was located at the N-terminus of Endostar. LC/MS was applied to verify the occurrence of mono-PEGylation at the N-terminus of Endostar.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13718508	Barium iodide		13718-50-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: