Home
Resources
Publications
Characterization of a New 1,3-1,4-β-glucanase Gene From Bacillus Tequilensis CGX5-1

Characterization of a New 1,3-1,4-β-glucanase Gene From Bacillus Tequilensis CGX5-1

Jinjing Wang, Chengtuo Niu, Xiaoling Liu, Xi Chen, Qi Li

Appl Biochem Biotechnol. 2014 Jun;173(3):826-37.

PMID: 24728764

Abstract:

1,3-1,4-β-Glucanase received great interest due to its application in brewing and feed industries. Application of 1,3-1,4-β-glucanase in brewing industry helps make up for the defect that plant-derived β-glucanases are heat-sensitive. A new strain, CGX5-1, exhibited remarkable 1,3-1,4-β-glucanase, was isolated from Asian giant hornet nest and identified Bacillus tequilensis. Moreover, a new 1,3-1,4-β-glucanase gene from B. tequilensis was cloned and measured to be 720 bp encoding 239 amino acids, with a predicted molecular weight of 26.9 kDa. After expressed in Escherichia coli BL21, active recombinant enzyme of 24 kDa was detected in the supernatant of cell culture, with the activity of 2,978.2 U/mL. The new enzyme was stable in the pH 5.0-7.5 with the highest activity measured at pH 6.0. Moreover, it is thermostable within 45 to 60 °C. The property of the new recombinant enzyme makes this enzyme a broad prospect in brewing industry. Moreover, this is the first report on 1,3-1,4-β-glucanase produced by B. tequilensis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP62213143	β-Glucanase 2, thermostable		62213-14-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: