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Characterization of a Novel Murine Preadipocyte Line, AP-18, Isolated From Subcutaneous Tissue: Analysis of Adipocyte-Related Gene Expressions

Characterization of a Novel Murine Preadipocyte Line, AP-18, Isolated From Subcutaneous Tissue: Analysis of Adipocyte-Related Gene Expressions

Chen Chen, Kazuma Takahashi, Ayumi Yoshida, Yumiko Takizawa, Yan Lee, Masaki Nakui, Hideyuki Doi, Yuji Takebayashi, Manabu Fukumoto, Testuya Yamada, Hideki Katagiri, Yoshitomo Oka, Jo Satoh

Cell Biol Int. 2010 Feb 22;34(3):293-9.

PMID: 19947910

Abstract:

Adipocyte lines are a useful tool for adipocyte research. Recently, a new preadipocyte line designated AP-18 was established from subcutaneous tissue of the C3H/He mouse. In this study, we further characterized AP-18 cells. Adipocyte differentiation was assessed by accumulation of fat droplets stained by Oil Red O. The expression of the preadipocyte- or adipocyte-specific genes and adipocytokine genes was analysed qualitatively by RT-PCR and quantitatively by real-time PCR in comparison with the LM cell, a murine fibroblast line, and the 3T3-L1 cell, respectively. AP-18 cells were fibroblastoid in maintenance culture. After the confluence, fat droplets were accumulated in 50-60% of the cells cultured in the medium alone and in 70-90% of the cells cultured with insulin within 2 to 3 weeks. The fat accumulation was not promoted by the addition of dexamethazone, IBMX (3-isobutyl-1-methylxanthine) or troglitazone in combination with insulin, which were obligatory for differentiation of the 3T3-L1 cell, a murine preadipocyte line. Throughout the differentiation, AP-18 cells expressed Pref-1, LPL, C/EBP beta, C/EBP delta, RXR alpha, C/EBP alpha, PPAR gamma, RXR gamma, aP2, GLUT4, SCD1, UCP2, UCP3, TNFalpha, resistin, leptin, adiponectin and PAI-1 genes, but not the UCP1 gene, indicating that the cell is derived from WAT (white adipose tissue). The time course of these gene expressions was similar to that of 3T3-L1 cells, although the expressions were slower and lower in AP-18 cells. These data indicate that AP-18 cells are preadipocytes originated from WAT and differentiate into adipocytes under more physiological conditions than 3T3-L1 cells. AP-18 may be useful in adipocyte research.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP55224947	AP-18		55224-94-7	Price

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