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Characterization of a Thermostable and Acidic-Tolerable Beta-Glucanase From Aerobic Fungi Trichoderma Koningii ZJU-T

Characterization of a Thermostable and Acidic-Tolerable Beta-Glucanase From Aerobic Fungi Trichoderma Koningii ZJU-T

J-L Wang, H Ruan, H-F Zhang, Q Zhang, H-B Zhang, G-Q He, S-R Shen

J Food Sci. 2007 Nov;72(9):C452-6.

PMID: 18034703

Abstract:

An extreme thermostable and acidic tolerable beta-glucanase was isolated and characterized from aerobic fungi Trichoderma koningii ZJU-T. The optimal reaction temperature and pH for the beta-glucanase were 100 degrees C and pH 2.0, respectively. The beta-glucanase showed increased stability at higher temperatures and lower pH values when compared to other beta-glucanases. The optimum conditions for the beta-glucanase stability were found to be pH 4.0 and 80 degrees C. Even subjected to 100 degrees C for 3 h, beta-glucanase activity did not show significant reduction. Moreover, K(+) significantly enhanced beta-glucanase activity at the concentration of 1 mM, while EDTA and other metal ions such as Mg(2+), Mn(2+), Zn(2+), Ca(2+), Fe(2+), Pb(2+), and Fe(3+) inhibited beta-glucanase activity. Denaturants, including sodium dodecyl sulfate (SDS) and mercaptoethanol, also inhibited beta-glucanase activity at a concentration of 5%. However, in the presence of 7 M urea, residual activity of the beta-glucanase still remained 14.5%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP62213143	β-Glucanase 2, thermostable		62213-14-3	Price

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