Home
Resources
Publications
Characterization of CYP1A in Hepatocytes of Cynomolgus Monkeys (Macaca Fascicularis) and Induction by Different Substituted Polychlorinated Biphenyls (PCBs)

Characterization of CYP1A in Hepatocytes of Cynomolgus Monkeys (Macaca Fascicularis) and Induction by Different Substituted Polychlorinated Biphenyls (PCBs)

A S Van Der Burght, A P Kreikamp, G J Horbach, W Seinen, M Van Den Berg

Arch Toxicol. 1998 Oct;72(10):630-6.

PMID: 9851678

Abstract:

Cynomolgus monkeys (Macaca fascicularis) have been used previously as a model to study effects on cytochrome P450 (CYP) regulation. Until now it has not been elucidated which CYP1A proteins are present in this primate species. The aim of this study was to characterize CYP1A in untreated hepatocytes of cynomolgus monkey using two specific CYP1A inhibitors (alpha-naphthoflavone and furafylline). The effect of different substituted polychlorinated biphenyls (PCBs) on CYP1A regulation was also studied in these hepatocytes. Small quantities of CYP1A2 have been identified in untreated hepatocytes. Northern blots showed the presence of a CYP1A mRNA in untreated hepatocytes, when hybridizations where performed with human CYP1A2 cDNA. Inhibitions with furafylline and alpha-naphthoflavone also suggested the presence of CYP1A2 properties. After induction with different PCBs, (probably) CYP1A1 mRNA and enzyme activity were induced in cynomolgus monkey hepatocytes. As expected, 2,3',4,4',5-PeCB (PCB no. 118), a mono-ortho substituted congener, was a potent CYP1A inducer but 2,2',3,4,4',5',5'-HpCB (PCB no. 180), a di-ortho and 2,2',3,4',5,5',6-HpCB (PCB no. 187), a tri-ortho substituted PCB, could induce CYP1A mRNA and enzyme activity in cynomolgus monkey hepatocytes as well.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP35065293-A	PCB No 180		35065-29-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: