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Characterization of Neuromedin U Like Immunoreactivity in Rat, Porcine, Guinea-Pig and Human Tissue Extracts Using a Specific Radioimmunoassay

J Domin, M A Ghatei, P Chohan, S R Bloom

Biochem Biophys Res Commun. 1986 Nov 14;140(3):1127-34.

PMID: 3778484

Abstract:

Two novel bioactive peptides termed neuromedin U-8 and neuromedin U-25 have recently been isolated from porcine spinal cord but nothing is known of their occurrence and molecular forms in other species. Following gel permeation chromatography, a specific radioimmunoassay detected only a single molecular form of neuromedin U-like immunoreactivity (NmU-LI) in rat, porcine and human central nervous system and gastrointestinal tract. Only guinea pig tissue extracts revealed two molecular forms of NmU-Li. Reverse phase high performance liquid chromatographic (HPLC) analysis demonstrated that porcine NmU-LI co-eluted with synthetic neuromedin U-25 standard. Human and rat NmU-LI however, was more hydrophobic on HPLC thus indicating species differences.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP98395767 Neuromedin U-25 porcine Neuromedin U-25 porcine 98395-76-7 Price
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