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Characterization of Procaine Metabolism as Probe for the Butyrylcholinesterase Enzyme Investigation by Simultaneous Determination of Procaine and Its Metabolite Using Capillary Electrophoresis With Electrochemiluminescence Detection

Characterization of Procaine Metabolism as Probe for the Butyrylcholinesterase Enzyme Investigation by Simultaneous Determination of Procaine and Its Metabolite Using Capillary Electrophoresis With Electrochemiluminescence Detection

Jipei Yuan, Jianyuan Yin, Erkang Wang

J Chromatogr A. 2007 Jun 22;1154(1-2):368-72.

PMID: 17507024

Abstract:

Capillary electrophoresis with electrochemiluminescene detection was used to characterize procaine hydrolysis as a probe for butyrylcholinesterase by in vitro procaine metabolism in plasma with butyrylcholinesterase acting as bioscavenger. Procaine and its metabolite N,N-diethylethanolamine were separated at 16 kV and then detected at 1.25 V in the presence of 5.0 mM Ru(bpy)(3)2+, with the detection limits of 2.4x10(-7) and 2.0x10(-8) mol/L (S/N=3), respectively. The Michaelis constant Km value was 1.73x10(-4) mol/L and the maximum velocity Vmax was 1.62x10(-6) mol/L/min. Acetylcholine bromide and choline chloride presented inhibition effects on the enzymatic cleavage of procaine, with the 50% inhibition concentration (IC50) of 6.24x10(-3) and 2.94x10(-4) mol/L.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP66239	Acetylcholine bromide		66-23-9	Price

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