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Characterization of the Exposed Carbohydrates on the Sheath Surface of in Vitro-Derived Brugia Pahangi Microfilariae by Analysis of Lectin Binding

Characterization of the Exposed Carbohydrates on the Sheath Surface of in Vitro-Derived Brugia Pahangi Microfilariae by Analysis of Lectin Binding

A Furman, L R Ash

J Parasitol. 1983 Dec;69(6):1043-7.

PMID: 6674454

Abstract:

Adult female Brugia pahangi were maintained metabolically active in vitro for up to 35 days in Click's medium supplemented with 10% horse serum. For the first 14 to 18 days microfilariae were released into culture. Although these in vitro-derived microfilariae were morphologically identical to in vivo-derived microfilariae, they could be differentiated by their characteristic of binding to a panel of fluorescein-conjugated lectins. The results suggest that maturation and release of microfilariae are correlated with glycosidic alterations on the sheath surface.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248441	Click's Medium			Price

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