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Characterization of Volatiles and Aroma-Active Compounds in Honeybush (Cyclopia Subternata) by GC-MS and GC-O Analysis

Characterization of Volatiles and Aroma-Active Compounds in Honeybush (Cyclopia Subternata) by GC-MS and GC-O Analysis

Maritha Le Roux, J Christel Cronje, Barend V Burger, Elizabeth Joubert

J Agric Food Chem. 2012 Mar 14;60(10):2657-64.

PMID: 22394400

Abstract:

Volatile organic compounds (VOCs) in fermented honeybush, Cyclopia subternata, were sampled by means of a high-capacity headspace sample enrichment probe (SEP) and analyzed by gas chromatography-mass spectrometry (GC-MS). Stereochemistry was determined by means of enantioselective GC-MS with derivatized β-cyclodextrin columns as chiral selectors. A total of 183 compounds, the majority of which are terpenoids (103; 56%), were identified by comparing their mass spectra and retention indices with those of reference compounds or tentatively identified by comparison with spectral library or literature data. Of these compounds, 37 were determined by gas chromatography-olfactometry (GC-O), using detection frequency (DF) and aroma extract dilution analysis (AEDA), to be odor-active (FD ≥ 2). (E)-β-Damascenone, (R/S)-linalool, (E)-β-damascone, geraniol, (E)-β-ionone, and (7E)-megastigma-5,7,9-trien-4-one were identified with the highest FD factors (≥512). The odors of certain compounds, that is, (6E,8Z)-megastigma-4,6,8-trien-3-one, (6E,8E)-megastigma-4,6,8-trien-3-one, (7E)-megastigma-5,7,9-trien-4-one, 10-epi-γ-eudesmol, epi-α-muurolol, and epi-α-cadinol, were perceived by GC-O assessors as typically honeybush-like.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP23726912	(E)-β-Damascone		23726-91-2	Price

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