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Chemically-specific dual/differential CARS Micro-Spectroscopy of Saturated and Unsaturated Lipid Droplets

Chemically-specific dual/differential CARS Micro-Spectroscopy of Saturated and Unsaturated Lipid Droplets

Claudia Di Napoli, Francesco Masia, Iestyn Pope, Cees Otto, Wolfgang Langbein, Paola Borri

J Biophotonics. 2014 Jan;7(1-2):68-76.

PMID: 23193020

Abstract:

We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (poly-unsaturated glyceryl trilinolenate, mono-unsaturated glyceryl trioleate and saturated glyceryl tricaprylate and glyceryl tristearate) in a rapid and label-free way. A systematic comparison of Raman spectra with CARS and D-CARS spectra was used to identify D-CARS spectral signatures which distinguish the disordered poly-unsaturated lipids from the more ordered saturated ones both in the CH-stretch vibration region and in the fingerprint region, without the need for lengthy CARS multiplex acquisition and analysis. D-CARS images of the lipid droplets at few selected wavenumbers clearly resolved the lipid composition differences, and exemplify the potential of this technique for label-free chemically selective rapid imaging of cytosolic lipid droplets in living cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14465680	Glyceryl trilinolenate		14465-68-0	Price

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