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Chemiluminescence Enzyme Immunoassay for Thyroxin With Use of Glucose Oxidase and a bis(2,4,6-trichlorophenyl)oxalate-fluorescent Dye System

Chemiluminescence Enzyme Immunoassay for Thyroxin With Use of Glucose Oxidase and a bis(2,4,6-trichlorophenyl)oxalate-fluorescent Dye System

H Arakawa, M Maeda, A Tsuji

Clin Chem. 1985 Mar;31(3):430-4.

PMID: 3882274

Abstract:

In this chemiluminescence enzyme immunoassay for thyroxin, glucose oxidase (EC 1.1.3.4) is the enzyme label and the bound and free fractions are separated by the double-antibody solid-phase technique. In the assay of enzyme activity, hydrogen peroxide generated from glucose substrate is measured by its chemiluminescence reaction with bis(2,4,6-trichlorophenyl)oxalate and 8-anilino-1-naphthalene-sulfonic acid. The measurable range of thyroxin is 2.5 to 200 micrograms/L. The coefficients of variation for thyroxin concentrations of 43.2 and 12.8 micrograms/L were 7.0% and 8.6% (intra-assay), and 6.4% and 12.3% (interassay), respectively. The proposed method is applicable to large-scale preliminary screening of neonates for congenital hypothyroidism, with samples consisting of dried blood spotted on filter paper.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1165919	Bis(2,4,6-trichlorophenyl) oxalate		1165-91-9	Price

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