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Chemiluminescent Enzyme Immunoassay for Alpha-Fetoprotein Using beta-D-galactosidase as Label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as Substrate

Chemiluminescent Enzyme Immunoassay for Alpha-Fetoprotein Using beta-D-galactosidase as Label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as Substrate

H Arakawa, T Ikegami, M Maeda, A Tsuji

J Biolumin Chemilumin. May-Jun 1993;8(3):135-9.

PMID: 7684183

Abstract:

We developed a sensitive chemiluminescent sandwich-type enzyme immunoassay (CL-EIA) of alpha-fetoprotein (AFP) using beta-D-galactosidase (beta-gal) as a label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as a substrate. The CL-EIA for AFP was performed using two monoclonal antibodies, one antibody is labelled with beta-gal, the other is coated onto the inside surface of a polystyrene tube. The detection limit for AFP was 0.5 ng/mL, equivalent to 10 pg/assay tube. The coefficient of variation for within and between assay imprecision were 2.0%-4.9% (n = 10) and 4.4%-9.8% (n = 5), respectively. AFP values in serum determined by this method correlated well with those obtained by radioimmunoassay (n = 26, r = 0.99). This sensitive AFP assay can be performed within 4 h and can be used as a routine assay in clinical diagnosis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7240906	5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside		7240-90-6	Price

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