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Chemiluminescent Enzyme Immunoassay Using beta-D-galactosidase as the Label and the bis(2,4,6-trichlorophenyl)oxalate-fluorescent Dye System

Chemiluminescent Enzyme Immunoassay Using beta-D-galactosidase as the Label and the bis(2,4,6-trichlorophenyl)oxalate-fluorescent Dye System

S Takayasu, M Maeda, A Tsuji

J Immunol Methods. 1985 Nov 7;83(2):317-25.

PMID: 2414365

Abstract:

A chemiluminescent enzyme immunoassay (EIA) using beta-D-galactosidase as the enzyme label is described in this report. The activity of beta-D-galactosidase, after separation of bound and free fractions, was measured using a coupled enzyme procedure: lactose and glucose oxidase were used as the substrate and coupling enzyme, respectively. Hydrogen peroxide generated from glucose was determined by the chemiluminescence reaction using the bis(2,4,6-trichlorophenyl)oxalate-fluorescent dye system. This assay system was applied to the EIA of phenytoin using beta-D-galactosidase as the label and assay sensitivity was increased about 10 times compared with the original method. This method may also be applied to other EIAs of digitoxin, alpha-fetoprotein and thyroid-stimulating hormone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1165919	Bis(2,4,6-trichlorophenyl) oxalate		1165-91-9	Price

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