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Chiral Separation of N-methyl-DL-aspartic Acid in Rat Brain Tissue as N-ethoxycarbonylated (S)-(+)-2-octyl Ester Derivatives by GC-MS

Chiral Separation of N-methyl-DL-aspartic Acid in Rat Brain Tissue as N-ethoxycarbonylated (S)-(+)-2-octyl Ester Derivatives by GC-MS

Duc-Toan Nguyen, Kyoung-Rae Kim, Gwang Lee, Man-Jeong Paik

Biomed Chromatogr. 2012 Nov;26(11):1353-6.

PMID: 22290726

Abstract:

A selective and sensitive analytical method was developed for enantiomeric separation and determination of N-methyl-DL-aspartic acid (NMA). The method involved the conversion of each enantiomer into N-ethoxycarbonylated (S)-(+)-2-octyl ester derivative for the direct separation by gas chromatography-mass spectrometry (GC-MS). The diastereomeric derivatives showed characteristic mass spectral properties for analysis by selected ion monitoring mode (SIM) and enabling enantioseparation on an achiral capillary column. Two enantiomers were baseline separated, and the detection limits for N-methyl-L-aspartic acid (NMLA) and N-methyl-D-aspartic acid (NMDA) were 0.07 and 0.03 ng/g, respectively. When applied to rat brain tissues for absolute configuration of NMA, only NMDA was determined, while NMLA was monitored as lower than the limit of detection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP68315355	DL-Aspartic acid-4-13C		68315-35-5	Price

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