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Chlorophyll a Is a Favorable Substrate for Chlamydomonas Mg-dechelatase Encoded by STAY-GREEN

Chlorophyll a Is a Favorable Substrate for Chlamydomonas Mg-dechelatase Encoded by STAY-GREEN

Kaori Matsuda, Yousuke Shimoda, Ayumi Tanaka, Hisashi Ito

Plant Physiol Biochem. 2016 Dec;109:365-373.

PMID: 27810676

Abstract:

Mg removal from chlorophyll by Mg-dechelatase is the first step of chlorophyll degradation. Recent studies showed that in Arabidopsis, Stay Green (SGR) encodes Mg-dechelatase. Though the Escherichia coli expression system is advantageous for investigating the properties of Mg-dechelatase, Arabidopsis Mg-dechelatase is not successfully expressed in E. coli. Chlamydomonas reinhardtii SGR (CrSGR) has a long, hydrophilic tail, suggesting that active CrSGR can be expressed in E. coli. After the incubation of chlorophyll a with CrSGR expressed in E. coli, pheophytin a accumulated, indicating that active CrSGR was expressed in E. coli. Substrate specificity of CrSGR against chlorophyll b and an intermediate molecule of the chlorophyll b degradation pathway was examined. CrSGR exhibited no activity against chlorophyll b and low activity against 7-hydroxymethyl chlorophyll a, consistent with the fact that chlorophyll b is degraded only after conversion to chlorophyll a. CrSGR exhibited low activity against divinyl chlorophyll a and chlorophyll a', and no activity against chlorophyllide a, protochlorophyll a, chlorophyll c2, and Zn-chlorophyll a. These observations indicate that chlorophyll a is the most favorable substrate for CrSGR. When CrSGR was expressed in Arabidopsis cells, the chlorophyll content decreased, further confirming that SGR has Mg-dechelating activity in chloroplasts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP479618-A	Chlorophyll a		479-61-8	Price

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