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Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans

Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans

Barbara Mulloy, John Hogwood

Methods Mol Biol. 2015;1229:105-18.

PMID: 25325948

Abstract:

Glycosaminoglycan samples are usually polydisperse, consisting of molecules with differing length and differing sequence. Methods for measuring the molecular weight of heparin have been developed to assure the quality and consistency of heparin products for medicinal use, and these methods can be applied in other laboratory contexts. In the method described here, high-performance gel permeation chromatography is calibrated using appropriate heparin molecular weight markers or a single broad standard calibrant, and used to characterize the molecular weight distribution of polydisperse samples or the peak molecular weight of monodisperse, or approximately monodisperse, heparin fractions. The same technology can be adapted for use with other glycosaminoglycans.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9041081-G	Heparin Sodium Molecular Weight Calibrant		9041-08-1	Price
AP9041081-J	Low Molecular Weight Heparin Molecular Weight Calibrant		9041-08-1	Price

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