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Chrysamine-G, a Lipophilic Analogue of Congo Red, Inhibits A Beta-Induced Toxicity in PC12 Cells

Chrysamine-G, a Lipophilic Analogue of Congo Red, Inhibits A Beta-Induced Toxicity in PC12 Cells

W E Klunk, M L Debnath, A M Koros, J W Pettegrew

Life Sci. 1998;63(20):1807-14.

PMID: 9820124

Abstract:

Increasing evidence suggests that deposition of amyloid-beta (A beta) peptide leads to neurodegeneration in Alzheimer's disease. Congo red, a histologic dye that binds to amyloid has previously been shown to diminish the toxic effects of A beta in cell culture. Since Congo red is too highly charged to enter the brain in significant quantities, a lipophilic derivative, Chrysamine-G, was tested for the ability to attenuate A beta[25-35]-induced toxicity in PC12 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Chrysamine-G showed a concentration-dependent inhibition of A beta[25-35]-induced toxicity. This protective effect became significant at 0.2 microM, a concentration very close to the Ki for Chrysamine-G binding to synthetic A beta (0.37 microM). A decarboxy derivative of Chrysamine-G, which does not bind to A beta, also did not protect against A beta-induced toxicity. The protective effects of Chrysamine-G may relate to its ability to bind directly to A beta and may involve other post-binding effects as well.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6472919	Chrysamine G		6472-91-9	Price

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