Home
Resources
Publications
Cinnamomum Verum Ingredient 2-methoxycinnamaldehyde: A New Antiproliferative Drug Targeting Topoisomerase I and II in Human Lung Squamous Cell Carcinoma NCI-H520 Cells

Cinnamomum Verum Ingredient 2-methoxycinnamaldehyde: A New Antiproliferative Drug Targeting Topoisomerase I and II in Human Lung Squamous Cell Carcinoma NCI-H520 Cells

Yi-Heng Liu, Kuen-Daw Tsai, Shu-Mei Yang, Ho-Yiu Wong, Ta-Wei Chen, Jonathan Cherng, Jaw-Ming Cherng

Eur J Cancer Prev. 2017 Jul;26(4):314-323.

PMID: 27192048

Abstract:

Cinnamomum verum has been used as a Chinese herbal medication. We investigated the antiproliferative effect of 2-methoxycinnamaldehyde (2-MCA), a constituent of the cortex of the plant, and the molecular biomarkers associated with tumorigenesis in human lung squamous cell carcinoma NCI-H520 cells. The effects of 2-MCA on cell growth, cytotoxicity, apoptosis, and topoisomerase I and II activities in human lung squamous cell carcinoma NCI-H520 cells were evaluated in vitro and in vivo. The results showed that 2-MCA inhibited proliferation and induced apoptosis as implicated by mitochondrial membrane potential (ΔΨm) loss, activation of both caspase 3 and caspase 9, as well as morphological characteristics of apoptosis. Furthermore, 2-MCA also induced lysosomal vacuolation with elevated volume of acidic compartment and cytotoxicity, and inhibited topoisomerase I as well as II activities. Additional study showed the antiproliferative effect of 2-MCA in a nude mice model. In short, our data imply that the antiproliferative activity of 2-MCA in vitro involved downregulation of cell growth markers, both topoisomerase I and II, and upregulation of proapoptotic molecules, associated with increased lysosomal vacuolation. In vivo, 2-MCA reduced the tumor size, which could have had a significant clinical impact. Our data imply that 2-MCA may be a potential agent for chemoprevention as well as anticancer therapy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1504741	2-Methoxycinnamaldehyde		1504-74-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: