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Citrus Auraptene Induces Glial Cell Line-Derived Neurotrophic Factor in C6 Cells

Citrus Auraptene Induces Glial Cell Line-Derived Neurotrophic Factor in C6 Cells

Yoshiko Furukawa, Ryu-Ichi Hara, Makiko Nakaya, Satoshi Okuyama, Atsushi Sawamoto, Mitsunari Nakajima

Int J Mol Sci. 2019 Dec 30;21(1):253.

PMID: 31905925

Abstract:

We previously demonstrated that auraptene (AUR), a natural coumarin derived from citrus plants, exerts anti-inflammatory effects in the brain, resulting in neuroprotection in some mouse models of brain disorders. The present study showed that treatment with AUR significantly increased the release of glial cell line-derived neurotrophic factor (GDNF), in a dose- and time-dependent manner, by rat C6 glioma cells, which release was associated with increased expression of GDNF mRNA. These results suggest that AUR acted as a neuroprotective agent in the brain via not only its anti-inflammatory action but also its induction of neurotrophic factor. We also showed that (1) the AUR-induced GDNF production was inhibited by U0126, a specific inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) 1/2, and by H89, a specific inhibitor of protein kinase A (PKA); and (2) AUR induced the phosphorylation of cAMP response element-binding protein (CREB), a transcription factor located within the nucleus. These results suggest that AUR-stimulated gdnf gene expression was up-regulated through the PKA/ERK/CREB pathway in C6 cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248902	Glial Cell Line-derived Neurotrophic Factor from rat			Price

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