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Cloning and Expression of a New Rat Procarboxypeptidase B Gene in Escherichia Coli and Purification of Recombination Carboxypeptidase B

Li Su-Xia, Zhang Yu-Jian, Tian Li-Ping, Yuan Qin-Sheng, Gong Yi

Protein Pept Lett. 2003 Dec;10(6):581-90.

PMID: 14683510

Abstract:

A new coding sequence of the procarboxypeptidase B gene was obtained from SD rat fresh pancreas by RT-PCR and highly expressed in Escherichia coli in inclusion bodies. The folded procarboxypeptidase B was subjected to trypsin enzymatic cleavage to produce active carboxypeptidase B, subsequently, carboxypeptidase B was effectively purified with anion exchange chromatography DEAE-FF and hydrophobic interaction chromatography Octyl FF, as a result, 40 mg carboxypeptidase B per litre cell culture with specific activity 7.42 u/mg was achieved. Further research showed that the obtained recombinant carboxypeptidase B could substitute carboxypeptidase B isolated from pancreas.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412297 CARBOXYPEPTIDASE-B rat CARBOXYPEPTIDASE-B rat Price
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