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[Cloning and Expression of Human Keratin 8 Gene cDNA in E.coli]

[Cloning and Expression of Human Keratin 8 Gene cDNA in E.coli]

Wei Li, Meng Xun, Yong-Lie Chu, Jian-Wu Zheng

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jan;26(1):41-3.

PMID: 20056087

Abstract:

Aim:
To clone and express human keratin 8 gene cDNA in E.coli.
Methods:
Human cytokeratin 8 gene cDNA was amplified by RT-PCR from genomic RNA of human cell line 7721. The amplified cytokeratin 8 gene cDNA was cloned into pMD18-T vector. Then, the CK8 cDNA was amplified by PCR from recombinant plasmid pMD18-CK8, and was subcloned into pET-28a(+) expression vector. The recombinant plasmid pET-28a-CK8 DNA was transformed into E.coli DH5alpha strain.
Results:
Human cytokeratin 8 gene cDNA was cloned, and the recombinant plasmid pMD18-CK8 was transformed into E.coli. The CK8 cDNA was subcloned into E.coli DH5alpha strain, and successfully expressed in E.coli.
Conclusion:
Human cytokeratin 8 gene cDNA is cloned, and successfully expressed in E.coli, which lay the foundation of further study on the CK8 biological properties and functions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241175	Keratin-8 human			Price

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