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Cloning and Expression of Human Neuron-Specific Enolase cDNA in Escherichia Coli

Cloning and Expression of Human Neuron-Specific Enolase cDNA in Escherichia Coli

K A Pavlov, O I Gurina, O M Antonova, A V Semenova, V P Chekhonin

Bull Exp Biol Med. 2011 Dec;152(2):206-9.

PMID: 22808461

Abstract:

cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42412131	Neuron-specific enolase from human brain			Price

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