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Cloning of Genomic DNA of Rhizopus Niveus Lipase and Expression in the Yeast Saccharomyces Cerevisiae

Cloning of Genomic DNA of Rhizopus Niveus Lipase and Expression in the Yeast Saccharomyces Cerevisiae

M Kohno, M Enatsu, W Kugimiya

Biosci Biotechnol Biochem. 1998 Dec;62(12):2425-7.

PMID: 9972270

Abstract:

Genomic DNA encoding Lipase I was cloned from Rhizopus niveus strain IFO4759. For expression of this gene in S. cerevisiae, DBY746 was transformed with YEp352PLipS, which had the cloned lipase gene under the control of a PGK promoter. This strain secreted the lipase at a high level (350 U/ml). The strain ND-12B was produced by a mating of DBY746, harboring YEp352PLipS, and NA74-3A, and dissection of asci. This new strain secreted the lipase up to 530 U/ml. Moreover, the lipase was produced most effectively in a medium containing Bacto-yeast extract, soy-peptide, and sucrose.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001621-G	Lipase from Rhizopus niveus		9001-62-1	Price

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