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Coexpression of an Intronic microRNA and Its Host Gene Reveals a Potential Role for miR-483-5p as an IGF2 Partner

Coexpression of an Intronic microRNA and Its Host Gene Reveals a Potential Role for miR-483-5p as an IGF2 Partner

Ning Ma, Xidi Wang, Yu Qiao, Fuyuan Li, Yang Hui, Chaoxia Zou, Jianfeng Jin, Guixiang Lv, Yahui Peng, Lujing Wang, Hui Huang, Lingyun Zhou, Xiaofei Zheng, Xu Gao

Mol Cell Endocrinol. 2011 Feb 10;333(1):96-101.

PMID: 21146586

Abstract:

MicroRNAs (miRNAs) are endogenous noncoding RNAs that downregulate gene expression by inhibiting translation or promoting mRNA degradation. Although over one-third of miRNAs are located within the intronic regions of transcription units, the expression of such "intron-derived" (or "intronic") miRNAs and their relationship with their host gene remain a mystery. Here, we show that miR-483-5p, which is located within the second intron of the insulin-like growth factor (Igf2) gene, is downregulated in mouse Hepa1-6 cells in a direct correlation with the Igf2 transcript by chromeceptin, an inhibitor of Igf2 at the transcriptional level. Furthermore, miR-483-5p overexpression and knockdown regulates the suppressor of cytokine signalling 3 (Socs3) and Igf2 mRNAs, respectively. Finally, Socs3, a key putative leptin-resistant factor in obesity, is identified as a direct target of miR-483-5p. These data suggest that miR-483-5p can be coexpressed together with its host gene, Igf2, and revealed the link between this miRNA and the IGF2 growth factor. In addition, these observations not only provide supporting evidence for the codependent expression of intronic miRNAs and their host genes in vitro, but also give insight into the role of miR-483-5p in metabolism regulation and tumourigenesis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP331859860	Chromeceptin		331859-86-0	Price

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