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Collaborative Action of M-CSF and CTGF/CCN2 in Articular Chondrocytes: Possible Regenerative Roles in Articular Cartilage Metabolism

Collaborative Action of M-CSF and CTGF/CCN2 in Articular Chondrocytes: Possible Regenerative Roles in Articular Cartilage Metabolism

Kyouji Nakao, Satoshi Kubota, Hideyuki Doi, Takanori Eguchi, Morihiko Oka, Takuo Fujisawa, Takashi Nishida, Masaharu Takigawa

Bone. 2005 May;36(5):884-92.

PMID: 15820145

Abstract:

It is known that expression of the macrophage colony-stimulating factor (M-CSF) gene is induced in articular chondrocytes upon inflammation. However, the functional role of M-CSF in cartilage has been unclear. In this study, we describe possible roles of M-CSF in the protection and maintenance of the articular cartilage based on the results of experiments using human chondrocytic cells and rat primary chondrocytes. Connective tissue growth factor (CTGF/CCN2) is known to be a potent molecule to regenerate damaged cartilage by promoting the growth and differentiation of articular chondrocytes. Here, we uncovered the fact that M-CSF induced the mRNA expression of the ctgf/ccn2 gene in those cells. Enhanced production of CTGF/CCN2 protein by M-CSF was also confirmed. Furthermore, M-CSF could autoactivate the m-csf gene, forming a positive feed-back network to amplify and prolong the observed effects. Finally, promotion of proteoglycan synthesis was observed by the addition of M-CSF. These findings taken together indicate novel roles of M-CSF in articular cartilage metabolism in collaboration with CTGF/CCN2, particularly during an inflammatory response. Such roles of M-CSF were further supported by the distribution of M-CSF producing chondrocytes in experimentally induced rat osteoarthritis cartilage in vivo.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413278	M-CSF from rat			Price

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