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Collagen/elastin Hydrogels Cross-Linked by Squaric Acid

Collagen/elastin Hydrogels Cross-Linked by Squaric Acid

J Skopinska-Wisniewska, J Kuderko, A Bajek, M Maj, A Sionkowska, M Ziegler-Borowska

Mater Sci Eng C Mater Biol Appl. 2016 Mar;60:100-108.

PMID: 26706512

Abstract:

Hydrogels based on collagen and elastin are very valuable materials for medicine and tissue engineering. They are biocompatible; however their mechanical properties and resistance for enzymatic degradation need to be improved by cross-linking. Up to this point many reagents have been tested but more secure reactants are still sought. Squaric acid (SqAc), 3,4-dihydroxy 3-cyclobutene 1,2-dione, is a strong, cyclic acid, which reacts easily with amine groups. The properties of hydrogels based on collagen/elastin mixtures (95/5, 90/10) containing 5%, 10% and 20% of SqAc and neutralized via dialysis against deionized water were tested. Cross-linked, 3-D, transparent hydrogels were created. The cross-linked materials are stiffer and more resistant to enzymatic degradation than those that are unmodified. The pore size, swelling ability and surface polarity are reduced due to 5% and 10% of SqAc addition. At the same time, the cellular response is not significantly affected by the cross-linking. Therefore, squaric acid would be regarded as a safe, effective cross-linking agent.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2892515	3,4-Dihydroxy-3-cyclobutene-1,2-dione		2892-51-5	Price

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