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Colorimetric Detection of Acetyl Xylan Esterase Activities

Galina Mai-Gisondi, Emma R Master

Methods Mol Biol. 2017;1588:45-57.

PMID: 28417360

Abstract:

Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-D-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2747059 4-Methylumbelliferyl acetate 4-Methylumbelliferyl acetate 2747-05-9 Price
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