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Colorimetric Method for the Determination of Lipoxygenase Activity

G E Anthon, D M Barrett

J Agric Food Chem. 2001 Jan;49(1):32-7.

PMID: 11170556

Abstract:

A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP99649 3-(Dimethylamino)benzoic acid 3-(Dimethylamino)benzoic acid 99-64-9 Price
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