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Combination of Micelle Collapse and Field-Amplified Sample Stacking in Capillary Electrophoresis for Determination of Trimethoprim and Sulfamethoxazole in Animal-Originated Foodstuffs

Combination of Micelle Collapse and Field-Amplified Sample Stacking in Capillary Electrophoresis for Determination of Trimethoprim and Sulfamethoxazole in Animal-Originated Foodstuffs

Lihong Liu, Qian Wan, Xiaoying Xu, Shunshan Duan, Chunli Yang

Food Chem. 2017 Mar 15;219:7-12.

PMID: 27765261

Abstract:

An on-line preconcentration method combining micelle to solvent stacking (MSS) with field-amplified sample stacking (FASS) was employed for the analysis of trimethoprim (TMP) and sulfamethoxazole (SMZ) by capillary zone electrophoresis (CZE). The optimized experimental conditions were as followings: (1) sample matrix, 10.0mM SDS-5% (v/v) methanol; (2) trapping solution (TS), 35mM H3PO4-60% acetonitrile (CH3CN); (3) running buffer, 30mM Na2HPO4 (pH=7.3); (4) sample solution volume, 168nL; TS volume, 168nL; and (5) 9kV voltage, 214nm UV detection. Under the optimized conditions, the limits of detection (LODs) for SMZ and TMP were 7.7 and 8.5ng/mL, and they were 301 and 329 times better compared to a typical injection, respectively. The contents of TMP and SMZ in animal foodstuffs such as dairy products, eggs and honey were analyzed, too. Recoveries of 80-104% were acquired with relative standard deviations of 0.5-5.4%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7757826-A	Sodium sulfate anhydrous		7757-82-6	Price

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