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Combining Adhesive Nanostructured Surfaces and Costimulatory Signals to Increase T Cell Activation

Combining Adhesive Nanostructured Surfaces and Costimulatory Signals to Increase T Cell Activation

Judith Guasch, Marco Hoffmann, Jennifer Diemer, Hossein Riahinezhad, Stefanie Neubauer, Horst Kessler, Joachim P Spatz

Nano Lett. 2018 Sep 12;18(9):5899-5904.

PMID: 30088769

Abstract:

Adoptive cell therapies are showing very promising results in the fight against cancer. However, these therapies are expensive and technically challenging in part due to the need of a large number of specific T cells, which must be activated and expanded in vitro. Here we describe a method to activate primary human T cells using a combination of nanostructured surfaces functionalized with the stimulating anti-CD3 antibody and the peptidic sequence arginine-glycine-aspartic acid, as well as costimulatory agents (anti-CD28 antibody and a cocktail of phorbol 12-myristate 13-acetate, ionomycin, and protein transport inhibitors). Thus, we propose a method that combines nanotechnology with cell biology procedures to efficiently produce T cells in the laboratory, challenging the current state-of-the-art expansion methodologies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ISM6705	Calcium ionophore I - cocktail A			Price

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