Home
Resources
Publications
Comparative Hepatic Gene Expression Profiling of Rats Treated With 1H,1H,2H,2H-heptadecafluorodecan-1-ol or With Pentadecafluorooctanoic Acid

Comparative Hepatic Gene Expression Profiling of Rats Treated With 1H,1H,2H,2H-heptadecafluorodecan-1-ol or With Pentadecafluorooctanoic Acid

Anja Julie Nilsen, Maria A Landin, Kristin H Haug, Frode Fonnum, Urs Berger, Harald Osmundsen

Physiol Genomics. 2008 Aug 15;34(3):285-303.

PMID: 18544661

Abstract:

Pentadecafluorooctanoic acid is an established peroxisome proliferator. Little is known about effects of treatment with 1H,1H,2H,2H-heptadecafluorodecan-1-ol, which is metabolized to pentadecafluorooctanoic acid. We compared effects of various dosages (3, 10, or 25 mg/kg body wt) of each of these compounds on hepatic gene expression in rats with microarrays. Microarray data were validated by real-time RT-PCR. Expression data were also correlated with hepatic activities of selected enzymes and with hepatic levels of pentadecafluorooctanoic acid and 1H,1H,2H,2H-heptadecafluorodecan-1-ol. Pentadecafluorooctanoic acid caused the more powerful change in gene expression, in terms of both number of genes affected and extent of change in expression. Across the dosages used pentadecafluorooctanoic acid and 1H,1H,2H,2H-heptadecafluorodecan-1-ol caused significant (P < or = 0.05) changes in expression for 441 and 105 genes, respectively. With 1H,1H,2H,2H-heptadecafluorodecan-1-ol approximately 38% of the 105 genes exhibited decreased expression with a dose of 25 mg/kg body wt, these genes also appearing less responsive to treatment at the lower dosages. Bioinformatic analysis suggested that these genes are associated with regulatory functions. With pentadecafluorooctanoic acid, increasing dosage up to 10 mg/kg body wt brought about progressive increase in expression of affected genes. Pathways analysis suggested similar effects of the two compounds on lipid and amino acid metabolism. Marked differences were also found, particularly with respect to effects on genes related to oxidative phosphorylation, oxidative metabolism, free radical scavenging, xenobiotic metabolism, and complement and coagulation cascades.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP252237404-A	1H,1H,2H,2H-Perfluorooctanephosphonic acid		252237-40-4	Price
AP335671-A	Pentadecafluorooctanoic acid		335-67-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: