Home
Resources
Publications
Comparative Inhibitory Effects of 4-allylpyrocatechol Isolated From Piper Betle on Streptococcus Intermedius, Streptococcus Mutans, and Candida Albicans

Comparative Inhibitory Effects of 4-allylpyrocatechol Isolated From Piper Betle on Streptococcus Intermedius, Streptococcus Mutans, and Candida Albicans

Pimpak Phumat, Sakornrat Khongkhunthian, Phenphichar Wanachantararak, Siriporn Okonogi

Arch Oral Biol. 2020 May;113:104690.

PMID: 32155466

Abstract:

Streptococcus intermedius, Streptococcus mutans, and Candida albicans are harmful oral pathogens and prone to resist chemical antimicrobial agents. Active ingredients from plants are of increasing interest as an alternative. This study aims to compare antimicrobial effects of 4-allylpyrocatechol (APC) extracted from Piper betle on these oral pathogens. Minimum concentration of APC against the tested pathogens was determined using a broth microdilution assay. Killing kinetic study of APC was carried out within 24 h. Morphology of the pathogenic cells was assessed using scanning electron microscopy (SEM). Anti-biofilm was investigated using crystal violet assay and confocal laser scanning microscopy (CLSM). The results showed that the mechanism of inhibition of APC was bactericidal and fungicidal effects. APC at minimum concentration of 400 μg/mL could completely kill Streptococcus and Candida spp., however, the killing rate on S. intermedius and C. albicans was significantly faster than on S. mutans. APC inhibited biofilm formation of C. albicans more efficiently than that of the bacterial cells. Cell morphology from SEM indicated that APC caused bacterial cell membrane destruction and inhibited fungal budding or tubing formation. CLSM images confirmed the killing potential of APC and suggested that bacterial dead cells could be easier washed out than the fungal dead cells. It is concluded that APC potentially inhibits growth and biofilms of oral Streptococcus and Candida spp. in different mechanism of action and killing rate. APC can be considered as a promising agent for preventing and treating dental disorders caused by S. intermedius, S. mutans, and C. albicans.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1126610	4-Allylpyrocatechol		1126-61-0	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: