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Comparative Study on the Characterization of Antisera of Anti-Aflatoxin B1 From Rabbit and Laying Hen

Comparative Study on the Characterization of Antisera of Anti-Aflatoxin B1 From Rabbit and Laying Hen

Fusheng Chen, Qi Zhou

Nahrung. 2002 Dec;46(6):432-6.

PMID: 12577594

Abstract:

Polyclonal antisera specific for aflatoxin B1 (AFB1) were developed in rabbits and laying hens immunized with AFB1 conjugated to bovine serum albumin (AFB1-BSA). During the immunization schedule, AFB1-specific antisera from rabbits started to obviously be secreted after 60 days of the first AFB1-BSA injection, reached to a peak at day 120, and started to go down at day 135; while in regard to laying hens, antibodies began to largely be produced at day 90, arrived to a peak at day 135, and began to go down at day 165. The rabbits consistently produced anti-AFB1 antisera in 2-fold higher amounts than the laying hens during the antisera production process. But rabbits' and laying hens' antisera to AFB1 were found not to be apparently different in cross-reaction with aflatoxin analogs. Both rabbits' and laying hens' antisera could be used as immunological reagents for detecting AFB1 in agricultural commodities.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42416649	Anti-phospho-Cyclin B1 (pSer126) antibody produced in rabbit			Price

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