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Comparisons of the Pharmacokinetic and Tissue Distribution Profiles of Withanolide B After Intragastric Administration of the Effective Part of Datura Metel L. In Normal and Psoriasis Guinea Pigs

Comparisons of the Pharmacokinetic and Tissue Distribution Profiles of Withanolide B After Intragastric Administration of the Effective Part of Datura Metel L. In Normal and Psoriasis Guinea Pigs

Lianrong Yang, Xin Meng, Haixue Kuang

J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 15;1083:284-288.

PMID: 29574380

Abstract:

A simple, highly sensitive ultra-performance liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed to quantify of withanolide B and obakunone (IS) in guinea pig plasma and tissues, and to compare the pharmacokinetics and tissue distribution of withanolide B in normal and psoriasis guinea pigs. After mixing with IS, plasma and tissues were pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C18 column using aqueous (0.1% formic acid) and acetonitrile (0.1% formic acid) solutions at 0.4 mL/min as the mobile phase. The gradient program was selected (0-4.0 min, 2-98% B; 4.0-4.5 min, 98-2% B; and 4.5-5 min, 2% B). Detection was performed on a 4000 QTRAP UPLC-ESI-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Withanolide B and obakunone (IS) were monitored under positive ionization conditions. The optimized mass transition ion-pairs (m/z) for quantitation were 455.1/109.4 for withanolide B and 455.1/161.1 for obakunone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP56973412	Withanolide B		56973-41-2	Price

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