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Complete Genome of Bacillus subtilis subsp. subtilis KCTC 3135 T and Variation in Cell Wall Genes of B. subtilis Strains

Complete Genome of Bacillus subtilis subsp. subtilis KCTC 3135 T and Variation in Cell Wall Genes of B. subtilis Strains

Seonjoo Ahn, Sangmi Jun, Hyun-Joo Ro, Ju Han Kim, Seil Kim

J Microbiol Biotechnol. 2018 Oct 28;28(10):1760-1768.

PMID: 30441885

Abstract:

The type strain Bacillus subtilis subsp. subtilis KCTC 3135T was deeply sequenced and annotated, replacing a previous draft genome in this study. The tar and tag genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between B. s. subtilis and B. s. spizizenii. However, a comparative genomic analysis of B. subtilis spp. revealed that both B. s. subtilis and B. s. spizizenii had various types of cell walls. These tar and tag operons were mutually exclusive and the tar genes from B. s. spizizenii were very similar to the genes from non-Bacillus bacteria, unlike the tag genes from B. s. subtilis. The results and previous studies suggest that the tar genes and the tag genes are not inherited after subspecies speciation. The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on tar genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies. These findings indicate that the tar genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of B. subtilis and that the description of subspecies B. s. spizizenii should be updated.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42410691	Microbial DNA standard from Bacillus subtilis			Price

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