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Concomitant Preparative Isolation of Calmodulin and Heat Shock Protein (hsp90) From Bovine Testes

Concomitant Preparative Isolation of Calmodulin and Heat Shock Protein (hsp90) From Bovine Testes

L S Wright, K A Finn, F L Siegel

Protein Expr Purif. 1993 Oct;4(5):417-24.

PMID: 8251754

Abstract:

We have described a convenient procedure for isolating large amounts of both calmodulin and the 90-kDa heat shock protein (hsp90) from bovine testes. These two proteins coeluted from phenyl-Sepharose during calcium-dependent hydrophobic interaction chromatography. The hsp90 was separated from calmodulin by gel filtration, purified by reverse-phase HPLC or lectin affinity chromatography, and identified by N-terminal amino acid sequence and immunoblotting with hsp90-specific monoclonal antibody. The N-terminus of bovine hsp90 shared 93% amino acid identity with the murine alpha-isoform of hsp90. A third major testes protein of 97 kDa was also isolated with this protocol and was found to be homologous to the 94-kDa glucose-regulated protein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP77107461	Calmodulin from bovine testes		77107-46-1	Price

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