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[Construction of a Family of Artificial Genes of Human Insulin]

V A Efimov, A A Buriakova, I N Pashkova, N N Polushin, O G Chakhmakhcheva

Bioorg Khim. 1989 Aug;15(8):1070-7.

PMID: 2686651

Abstract:

Using oligonucleotide-directed mutagenesis and chemical-enzymatic DNA synthesis, genes for A and B insulin chains, C-peptide and Tyr-C-peptide have been constructed starting from synthetic gene for human proinsulin synthesized earlier. The genes for human preproinsulin, mini-proinsulin, single-chain insulin and their modifications were also synthesized. The constructions obtained were cloned in plasmid vectors.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP139532119 Tyr-C-Peptide human Tyr-C-Peptide human 139532-11-9 Price
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