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Construction of an Enzymatic Route Using a Food-Grade Recombinant Bacillus Subtilis for the Production and Purification of Epilactose From Lactose

Construction of an Enzymatic Route Using a Food-Grade Recombinant Bacillus Subtilis for the Production and Purification of Epilactose From Lactose

Qiuming Chen, Weiwei He, Xin Yan, Tao Zhang, Bo Jiang, Timo Stressler, Lutz Fischer, Wanmeng Mu

J Dairy Sci. 2018 Mar;101(3):1872-1882.

PMID: 29290443

Abstract:

Lactose is a main by-product in the cheese industry. Many attempts have been made to convert the lactose to high value-added products, including epilactose. Epilactose is a valuable prebiotic and can be epimerized from lactose with cellobiose 2-epimerase (CEase). The objective of the present work was to construct a food-grade recombinant Bacillus subtilis that produces CEase from Thermoanaerobacterium saccharolyticum. The CEase was expressed in B. subtilis without antibiotic resistance genes. After fermentation, the maximum volumetric activity of the fermented broth was more than 7 U/mL. The activity of the recombinant B. subtilis was increased by up to 3.7 fold after ethanol permeabilization. Then, 66.9 ± 0.7 g/L of epilactose was produced from 300 g/L of whey powder solution in 1 h with 13.3 U/mL of permeabilized biocatalyst. In addition, an enzymatic route including degradation of the lactose, yeast fermentation, and cation exchange chromatography was described to further purify the produced epilactose from lactose. Finally, epilactose with a purity >98% was produced from 300 g/L of lactose with a yield of 24.0%. In conclusion, neither antibiotics nor pathogenic bacteria were used throughout the epilactose production and purification procedure.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP50468569	Epilactose		50468-56-9	Price

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