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Construction of Aspergillus Niger Integrated With Cellulase Gene From Ampullaria Gigas Spix for Improved Enzyme Production and Saccharification of Alkaline-Pretreated Rice Straw

Construction of Aspergillus Niger Integrated With Cellulase Gene From Ampullaria Gigas Spix for Improved Enzyme Production and Saccharification of Alkaline-Pretreated Rice Straw

Peizhou Yang, Haifeng Zhang, Lili Cao, Zhi Zheng, Shaotong Jiang

3 Biotech. 2016 Dec;6(2):236.

PMID: 28330308

Abstract:

Aspergillus niger is an important microorganism that has been used for decades to produce extracellular enzymes. In this study, a novel Aspergillus niger strain integrated with a eukaryotic expression vector harboring the gpd-Shi promoter of shiitake mushrooms and cellulase gene of Ampullaria gigas Spix was engineered to improve cellulase production for the achievement of highly efficient saccharification of agricultural residues. In one strain, designated ACShi27, which exhibited the highest total cellulase expression, total cellulase, endoglucanase, exoglucanase, and xylanase expression levels were 1.73, 16.23, 17.73, and 150.83 U ml-1, respectively; these values were 14.5, 22.3, 24.6, and 17.3% higher than those of the wild-type Aspergillus niger M85 using wheat bran as an induction substrate. Production of cellulases and xylanase by solid-state fermentation followed by in situ saccharification of ACShi27 was investigated with alkaline-pretreated rice straw as a substrate. After 2 days of enzyme induction at 30 °C, followed by 48 h of saccharification at 50 °C, the conversion rate of carbon polymers into reducing sugar reached 293.2 mg g-1, which was 1.23-fold higher than that of the wild-type strain. The expression of sestc in Aspergillus niger can improve the total cellulase and xylanase activity and synergism, thereby enhancing the lignocellulose in situ saccharification.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9012548-A	Cellulase from Aspergillus niger		9012-54-8	Price

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