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Contemporaneous 3D Characterization of Acute and Chronic Myocardial I/R Injury and Response

Contemporaneous 3D Characterization of Acute and Chronic Myocardial I/R Injury and Response

Simon F Merz, Sebastian Korste, Lea Bornemann, Lars Michel, Pia Stock, Anthony Squire, Camille Soun, Daniel R Engel, Julia Detzer, Holger Lörchner, Dirk M Hermann, Markus Kamler, Joachim Klode, Ulrike B Hendgen-Cotta, etc.

Nat Commun. 2019 May 24;10(1):2312.

PMID: 31127113

Abstract:

Cardioprotection by salvage of the infarct-affected myocardium is an unmet yet highly desired therapeutic goal. To develop new dedicated therapies, experimental myocardial ischemia/reperfusion (I/R) injury would require methods to simultaneously characterize extent and localization of the damage and the ensuing inflammatory responses in whole hearts over time. Here we present a three-dimensional (3D), simultaneous quantitative investigation of key I/R injury-components by combining bleaching-augmented solvent-based non-toxic clearing (BALANCE) using ethyl cinnamate (ECi) with light sheet fluorescence microscopy. This allows structural analyses of fluorescence-labeled I/R hearts with exceptional detail. We discover and 3D-quantify distinguishable acute and late vascular I/R damage zones. These contain highly localized and spatially structured neutrophil infiltrates that are modulated upon cardiac healing. Our model demonstrates that these characteristic I/R injury patterns can detect the extent of damage even days after the ischemic index event hence allowing the investigation of long-term recovery and remodeling processes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP103366	Ethyl cinnamate		103-36-6	Price

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