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Continuous Photometric Determination of endo-1,4-beta-D-glucanase (Cellulase) Activity Using 4-methylumbelliferyl-beta-D-cellobioside as a Substrate

Continuous Photometric Determination of endo-1,4-beta-D-glucanase (Cellulase) Activity Using 4-methylumbelliferyl-beta-D-cellobioside as a Substrate

V M Chernoglazov, A N Jafarova, A A Klyosov

Anal Biochem. 1989 May 15;179(1):186-9.

PMID: 2757193

Abstract:

A very simple and sensitive procedure for the determination of the activity of highly purified endo-1,4-beta-glucanase from the microscopic fungus Trichoderma reesei using 4-methylumbelliferyl-beta-D-cellobioside has been developed. The HPLC study has shown that this substrate is cleaved by endo-1,4-beta-glucanase to form predominantly free 4-methylumbelliferone, Km and kcat being 1.25 mM and 7.9 s-1, respectively (30 degrees C, pH 5.0). The possibility of continuous photometric determination of the enzyme using the difference absorptivity coefficient of 1600 M-1 cm-1 at 350 nm has been demonstrated.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP72626610	4-Methylumbelliferyl β-D-cellobioside		72626-61-0	Price

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