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Copper(II) Complexes With Fusobacterium Nucleatum Adhesin FadA: Coordination Pattern, Physicochemical Properties and Reactivity

Copper(II) Complexes With Fusobacterium Nucleatum Adhesin FadA: Coordination Pattern, Physicochemical Properties and Reactivity

Katarzyna Krupa, Monika Lesiów, Kamila Stokowa-Sołtys, Radosław Starosta, Natalia Ptaszyńska, Anna Łęgowska, Krzysztof Rolka, Maciej Wernecki, Magdalena Cal, Małgorzata Jeżowska-Bojczuk

J Inorg Biochem. 2018 Dec;189:69-80.

PMID: 30243120

Abstract:

Fusobacterium nucleatum is an anaerobic, Gram-negative bacteria linked to colon cancer. It is interesting to determine how metal ions interact with bacterial adhesin proteins. To this end, the coordination of ATDAAS-NH2 and MKKFL-NH2 fragments of Fusobacterium adhesin A (FadA) to copper(II) ions was studied by potentiometry, spectroscopic techniques (UV-Vis, CD, EPR and NMR) and the density functional theory (DFT) methods. At pH 6.8 (colon physiological pH), the metal ion in the first peptide (ATDAAS-NH2) is coordinated by one oxygen and three nitrogen donors while in the second one (MKKFL-NH2) - by sulfur and three nitrogen atoms. Both complexes form two five- and one six-membered stable chelate rings. Moreover, reactivity studies confirmed the production of reactive oxygen species such as hydroxyl radical, superoxide anion radical and singlet oxygen. Generation of reactive oxygen species (ROS) was observed during gel electrophoresis and spectroscopic assays with reporting molecules like NDMA (N,N-dimethyl-p-nitrosoaniline) and NBT (Nitrotetrazolium Blue Chloride). All reactions were conducted in the presence of hydrogen peroxide as endogenous oxidant.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP127615605	3-Nitrotetrazolium blue chloride		127615-60-5	Price

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