Home
Resources
Publications
Copper-zinc Alloy Nanoparticle Based Enzyme-Free Superoxide Radical Sensing on a Screen-Printed Electrode

Copper-zinc Alloy Nanoparticle Based Enzyme-Free Superoxide Radical Sensing on a Screen-Printed Electrode

Burak Derkus, Emel Emregul, Kaan C Emregul

Talanta. 2015 Mar;134:206-214.

PMID: 25618659

Abstract:

In this paper, amperometric enzyme-free sensors using superoxide dismutase (SOD) enzyme as a catalyst for the dismutation reaction of superoxides into oxygen and hydrogen peroxide, enabling superoxide radical detection have been described. For this purpose, the surfaces of screen-printed platinum electrodes have been modified with gelatin composites of CuO, ZnO and CuZn nanoparticles with the expectation of an increase in catalytic effect toward the dismutation reaction. SOD containing electrodes were also prepared for comparative studies in which glutaraldehyde was used as a cross-linker for the immobilization of SOD to the nanocomposite materials. Electrochemical measurements were carried out using a screen-printed electrochemical system that included potassiumferrocyanide (K4[Fe(CN)6]) and potassiumferricyanide (K3[Fe(CN)6]) as the redox probes. The results revealed that the enzyme-free detection method using CuZn nanoparticles can determine superoxide radicals with high performance compared to other detection methods prepared with different nanoparticles by mimicking the active region of superoxide dismutase enzyme. The anodic (ks(a)) and cathodic (ks(c)) electron transfer rate constants and the anodic (α(a)) and cathodic (α(c)) transfer coefficients were evaluated and found to be ks(a)=6.31 s(-1) and α(a)=0.81, ks(c)=1.48 s(-1) and α(c)=0.19 for the gelatin-CuZn-SOD electrode; ks(a)=6.15 s(-1) and α(a)=0.79, ks(c)=1,63 s(-1) and α(c)=0.21 for the enzyme-free gelatin-CuZn electrode. The enzyme-free electrode showed nearly 80% amperometric performance with respect to the enzyme containing electrode indicating the superior functionality of enzyme-free electrode for the detection of superoxide radicals.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS76609	Copper-zinc alloy			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: